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KMID : 0380620180500050517
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Korean Journal of Food Science and Technology
2018 Volume.50 No. 5 p.517 ~ p.527
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Protective effect of ethyl acetate fraction from Actinidia arguta sprout against high glucose-induced in vitro neurotoxicity
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Yoo Seul-Ki
Park Seon-Kyeong
Kim Jong-Min
Kang Jin-Yong
Park Su-Bin
Han Hye-Ju
Kim Chul-Wo
Lee Uk
Heo Ho-Jin
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Abstract
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The current study investigated in vitro anti-diabetic and neuroprotective effects of the ethyl acetate fraction in Actinidia arguta sprouts (EFAS), on H2O2 and high glucose-induced cytotoxicity in human neuroblastoma MC-IXC cells. EFAS had high total phenolic and total flavonoid contents. An assessment of 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) radical scavenging activity of EFAS, as well as its potential for inhibiting malondialdehyde production, indicated that EFAS may possess significant antioxidant properties. EFAS exerted inhibitory effects on α-glucosidase via glycemic regulation which forms advanced glycation end products. In addition, EFAS exhibited significant acetylcholinesterase inhibitory effects. Moreover, EFAS displayed protective effects against H2O2 and high glucose-induced cell death, and inhibited the generation of reactive oxygen species in MC-IXC cells. Finally, the main physiological compound of EFAS was identified via high performance liquid chromatography as a rutin.
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KEYWORD
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Actinidia arguta, antioxidant effect, anti-diabetic effect, neuroprotective effect, MC-IXC cell
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